TEMPERATURE-GRADIENT PLATES FOR GROWTH OF MICROORGANISMS

Temperaturegradient plates for growth of microorganisms.

Landman, Otto E. (Fort Detrick, Frederick, Md.), Howard T. Bausum, and Thomas S. Matney. Temperature-gradient plates for growth of microorganisms. J. Bacteriol. 83:463-469. 1962.-Different temperature-gradient plates have been devised for the study of microbial growth on solid media through continuous temperature ranges or in liquid media at finely graded temperatures. All plates are made of he...

Temperature gradient chamber for relative growth rate analysis of yeast.

Relative growth is often used as a phenotypic measure to distinguish mutant and wild-type yeast or bacterial strains. Differential growth as a function of temperature is a convenient and accurate means of analyzing differences between strains. Slight differences in the genotypes of two strains frequently result in differential growth of the two strains as a function of temperature. We have deve...

Residue of horse serum purification as a nitrogen source for growth of microorganisms, l-Clostridia

3 Growth of Microorganisms

Detection of extracellular proteases from microorganisms on agar plates.

We present herein an improved assay for detecting the presence of extracellular proteases from microorganisms on agar plates. Using different substrates (gelatin, BSA, hemoglobin) incorporated into the agar and varying the culture medium composition, we were able to detect proteolytic activities from Pseudomonas aeruginosa, Micrococcus luteus and Serratia marcescens as well as the influence tha...